GPC cleanup is a size exclusion cleanup procedure for the post-extraction cleanup of high molecular weight interfering substances from environmental samples, food products, and animal tissues prior to analysis. Removal of contaminants leads to more reliable results, fewer sample repeats, and less downtime for downstream chromatography instruments.
Based on size exclusion chromatography (SEC), our VERITY® GPC Cleanup System discards high molecular weight interfering substances from sample extracts while recovering analytes of interest into a pure fraction of solvent to reduce the sample matrix effect significantly:
To improve the accuracy and sensitivity of the analytical results
To extend GC or LC column life and reduce analytical downtime
This cleanup technique is typically performed before analysis of contaminants and residues to remove lipids, proteins, cell debris, pigments, humic acid, and more, from animal tissue, fatty foods, plant tissue, soils sediment, sludge, and wastewater.
A Guide to Sample Cleanup Using Gel Permeation Chromatography (GPC)
Residue analysis workflows are common in a variety of laboratory and clinical settings. These workflows include testing for pesticides, antibiotics, fungicides, heavy metals, and a variety of other chemical or biological contaminants. Before these labs can directly test samples for contaminants, the samples must be processed so residues are first extracted. Then the post-extraction cleanup allows the removal of co-extracted contaminants to enable effective analysis without risk to expensive analytical equipment. In this article, we provide an overview of GPC and how it’s used for post-extraction cleanup
The VERITY® GPC Cleanup System is an ideal solution for post-extraction cleanup of high-molecular-weight interfering substances from environmental samples, food products, and animal tissue before analysis using gel permeation chromatography (GPC). Removing these contaminants leads to more reliable results, fewer sample repeats, and less downtime for downstream chromatography instruments. The system removes substances such as lipids, proteins, cell debris, pigments, humic acid, and more that can interfere with the analysis of pharmaceuticals and residues from animal tissue, fatty foods, plant tissue, soils sediment, sludge, and wastewater.
VERITY® GPC Cleanup System with optional VERITY® 1741 UV-VIS Detector can be used in cleanup procedures that use organic solvents for the mobile phase and a specialized column packed with a divinylbenzene-styrene copolymer. This method is recommended for the postextraction elimination of lipids, polymers, copolymers, proteins, natural resins, cellular components, and other high molecular weight compounds from the analytes of interest. The UV detector can be utilized by laboratories complying with GPC post-extraction guidelines set by the U.S. Environmental Protection Agency. The UV detector calibrates the GPC column, ensuring regulation standards.
