Gilson Connect
Quantitative polymerase chain reaction (qPCR) is a powerful and highly sensitive method of amplifying genetic material, with applications ranging from diagnostics and forensics to research.
However, ensuring the accuracy and reliability of your qPCR experiments can be challenging — even the smallest amount of contamination or failure to adhere strictly to liquid handling best practices can substantially impact your results.
So, what can you do to maximize your chances of delivering highly accurate qPCR results, time after time? In this article, we share our top 5 tips for qPCR success.
The manual cyclization step in a peptide purification workflow is time-consuming and can easily become the rate-limiting step of the whole workflow, leading to delays in peptide production. Automating a drop-wise cyclization process using a liquid handler with a large rack capacity and incorporating automatic orbital shaking is an ideal solution to this workflow bottleneck. Learn more in this technical note.
In this technical note, the use of a PIPETMAX® automated liquid handling system is described for the pipetting of the plasma from the whole blood sample. PIPETMAX can be used to aspirate a precise volume of the plasma and transfer this to a suitable analysis or storage tube. If the specific laboratory workflow will benefit from it, then diluent can also be added to the analysis tubes, and the plasma can be mixed into the diluent as part of the same method.
Switching from a manual to an automated process not only enables significantly more effective use of personnel time, but also removes operator error for reproducible results and increases the accuracy and precision of assays. In this article, we delve into the challenges that can arise during this transition and explain how Gilson's solutions and services can ease the process of transferring your manual methods to an automated system.
The vinegar fly, or fruit fly, is an excellent animal model for studying the underlying mechanisms of human disease. In preparation for use in research studies, fruit fly larvae must eat and grow continuously over a four-day period before hatching. In this app note, we describe the time-consuming and labor-intensive process of preparing vessels with fly food and how to avoid it using a fully automated system.
Did you know that about 75% of known human disease genes have a recognizable match in the genome of the fruit fly? In this technical note, a lab uses the Gilson Fly Food Dispenser to dispense fly food into vials at regular intervals.
The quantitative polymerase chain reaction (qPCR) has transformed the world of molecular biology methodology by enabling fast, high-throughput detection and quantification of DNA and RNA in real time. PIPETMAX is specifically designed to maximize reproducibility and accuracy in qPCR workflows. Here, we explore three ways that PIPETMAX can help you run more effective and efficient experiments.
qPCR plays a vital role in addressing today’s healthcare questions, with applications ranging from gene expression analysis to mutagenesis studies, and contamination is a significant concern. So, how can you ensure the integrity of your qPCR samples? This article explores where contamination arises from, and how to identify it. To improve sample integrity, we highlight approaches you can take to minimize contamination.
Central Nervous System (CNS) depressants can introduce calm and induce sleep. Benzodiazepines are CNS depressants used to relieve anxiety, insomnia, and panic attacks. This application note discusses a simple SPE urine extraction before LC/MS-MS to confirm the presence of 15 spiked benzodiazepine analytes.
The Coomassie (Bradford) assay is a well-established method for protein quantification that utilizes a commonly placed UV/Vis spectrophotometer. Streamlining the manual pipetting steps through
automated liquid handling results in less data variability and improvements in workflow efficiencies.
