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In this study, functional RNA was quantitated by RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System after two different extraction methods. To enhance the pipetting accuracy and consistency, PIPETMAX 268 was used to reduce pipetting errors inherent in manual methods.
Integrating automated liquid handling systems, like PIPETMAX® 268, into your RT-qPCR workflows for detecting SARS-CoV-2 can help remove the risk of manual errors in sample transfers.
How to Stay Safe and Comfortable in High-Pressure Lab Environments
In this study, functional corn RNA was quantitated by RT-qPCR using the GoTaq® Probe 1-Step RT-qPCR System after two different extraction methods. To enhance the pipetting accuracy and consistency an automated pipetting assistant, PIPETMAX 268 was used to reduce pipetting errors inherent in manual methods.
Normalization of the concentration of nucleic acid samples is a crucial yet tedious process that requires constant readjustment of the volume of the pipetting device. PIPETMAX® 268 Normalization Assistant automates this process: calculations are carried out by the software, eliminating arithmetic errors, and pipetting volumes are adjusted automatically - learn more in this app note.
Gene expression studies are often used to gain insight into complex interactions between organisms, including those between plants and viruses. Real-time PCR (qPCR) is a tool frequently used in these studies given its wide dynamic range, sensitivity, and ease of automation. Learn how PIPETMAX 268 can be used to automate qPCR sample prep in this informative app note.
Reagents used in qPCR master mixes, including buffers, surfactants, and detergents, can negatively influence accuracy and precision, thereby increasing sample variability and decreasing data reliability. In this app note, we used PIPETMAX® 268 to test whether the number of times a tip was pre-wet before transferring master mix affected qPCR performance.
This application note describes how PIPETMAX® 268 with qPCR Assistant is a highly proficient pipetting workstation that reduces the potential for cross-contamination and other human induced errors.
Quantitative real time PCR, often coupled with reverse transcription, is a very sensitive technique that can be used for many applications including gene expression analysis. PIPETMAX 268 Normalization Assistant delivers an easy-to-use software and hardware solution to automate normalization of sample concentration without compromising RNA integrity.
To make the qPCR technique more reproducible, scientists need liquid handling protocols that can be readily automated, modified, and transferred between different instruments without affecting the quality of the data itself.
